Animation: DNA sequencing – the Sanger method
This animation on the most commonly used method of sequencing DNA – the dideoxy or chain termination method
The dideoxy, or chain termination, method was developed by Fred Sanger in 1977, and he won his second Nobel Prize for the discovery.
In this method, the DNA is used as a template to generate a set of fragments that differ in length from each other by a single base. The fragments are then separated by size, and the bases at the end are identified, recreating the original sequence of the DNA.