Animation: DNA sequencing – the Sanger method

This animation on the most commonly used method of sequencing DNA – the dideoxy or chain termination method 

The dideoxy, or chain termination, method was developed by Fred Sanger in 1977, and he won his second Nobel Prize for the discovery.

In this method, the DNA is used as a template to generate a set of fragments that differ in length from each other by a single base. The fragments are then separated by size, and the bases at the end are identified, recreating the original sequence of the DNA.


About this resource

This resource was first published in ‘Genes, Genomes and Health’ in January 2010.

Genetics and genomics
Genes, Genomes and Health
Education levels:
16–19, Continuing professional development